Coding

Part:BBa_K2029006

Designed by: Niklas Schmacke, Max Schelski   Group: iGEM16_UBonn_HBRS   (2016-10-14)


BsCel5

Part coding for catalytic enzyme cellulase, which cleaves 1,4-beta-D-glycosidic linkages in cellulose. This specific gene sequence is naturally found in some strains of Bacillus subtilis.

Usage and Biology

The enzymes expressed can be used to hydrolyse cellulose molecules into simple sugars.

This gene is intended for use in B. subtilis, preceded by a secretory tag like BBa_K2029000 or BBa_K2029001 which signals the B. subtilis cell to secrete the coded protein into the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None